什么是Good's缓冲液
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一、Good's 缓冲液特点
Good's 缓冲液又称为两性离子缓冲液。1960年，N.E.Good和他的同事们总结了现有的各种缓冲试剂的优缺点后认为，必须用人为设计和人工合成的方法来找到专门用于生命科学研究的特定的缓冲体系，这些缓冲体系应具有以下特性：
1、pKa值在6-8之间；
2、在水中的溶解度高；
3、不易穿透生物膜；
4、盐效应小；
5、离子浓度、溶液组成和温度对解离的影响小；
6、不与金属离子生成复合物或沉淀；
7、该缓冲剂化学稳定；
8、紫外和可见光波长范围内光吸收小；
9、易制得高纯度的盐。
Good's缓冲液的主要优点：
不参加和不干扰生物化学反应过程，对酶化学反应等无抑制作用，所以它们专门用于细胞器和极易变性的、对pH敏感的蛋白质和酶的研究工作。
二、常用Good's缓冲剂PH使用范围
化学名称缩写词分子量pKa使用pH值范围2-(N-吗啉代)乙磺酸MES195.26.15.5～6.7二(2-羟乙基)亚氨基Tris(羟甲基)甲烷Bis-Tris109.26.55.8～7.2N(2-乙酰氨基)-2-亚氨基双乙酸ADA190.26.66.0～7.22[(2-氨基-2氧代乙基)氨基]乙磺酸ACES182.26.86.1～7.5哌嚓-N,N-二(2-乙磺酸)PIPES302.46.86.1～7.53-(N-吗啉代)-2-羟基丙磺酸MOPSO225.36.96.2～7.61,3-二[Tri(羟甲基)甲基氨基]丙烷Bis-Tris Propane282.36.86.3～9.5N,N-二(2-羟乙基)-2-氨基乙磺酸BES213.27.16.4～7.83-(N-吗啉代)丙磺酸MOPS209.37.26.5～7.9N-(2-羟乙基)哌嚓-N/-(2-乙磺酸)HEPES238.37.56.8～8.2N-tris(羟甲基)甲基-2-氨基乙磺酸TES229.27.46.8～8.23-[N,N-二(羟乙基)氨基]-2-羟基丙磺酸DIPSO243.37.67.0～8.23-[N-tris(羟甲基)甲基氨基]-2-羟基丙磺酸TAPSO259.37.67.0～8.2Tris(羟甲基)氨基甲烷TRIZAMA121.18.17.0～9.1N-(2-羟乙基)哌嗪-N/-(2-羟基丙磺酸)HEPPSO268.37.87.1～8.5哌嚓-N,N/-二(2-羟基丙磺酸)POPSO362.47.87.2～8.5N-(2-羟乙基)哌嚓-N/-(3-丙磺酸)EPPS252.387.3～8.7三乙醇胺TEA149.27.87.4～8.3N-tr诅(羟甲基)甲基甘氨酸Tricine179.28.17.4～8.8N,N-二(2-羟乙基)甘氨酸Bicine163.28.37.6～9.0N-tris(羟甲基)甲基-3-氨基丙磺酸TAPS243.38.47.7～9.13-[(1,1-二甲基-2-羟乙基)氨基]-2-羟基丙磺酸AMPSO227.398.3～9.72-(N-环己基氨基)乙磺酸CHES207.39.38.6～10.03-(环己基氨基)-2-羟基-1-丙磺酸CAPSO237.39.68.9～10.32-氨基-2-甲基-1-丙醇AMP89.19.79.0～10.53-(环己基氨基)-1-丙磺酸CAPS221.310.49.7～11.1伟伯化工可提供Good's缓冲液产品列表：
产品编号CAS编码产品描述300301210191-18-1N,N-双(2-羟乙基)-2-氨基乙磺酸, >98.0%(T)302961710191-18-1N,N-二(2-羟乙基)-2-氨基乙磺酸, 99%344686710191-18-1N,N-双(2-羟乙基）-2-氨基乙磺酸,3061377102-71-6三乙醇胺, 98+%3255031102-71-6三乙醇胺, VetecTM reagent grade, 97%3007062103-47-92-环己胺基乙磺酸, >99.0%(T)3035548103-47-92-(N-环己氨基)乙磺酸, 99%3446977103-47-92-(环己胺)乙磺酸,3460536103-47-92-(环己氨基)乙磺酸, 97+%30176361132-61-23-吗啉丙磺酸, >98.0%(T)30554271132-61-23-(N-吗啉)丙磺酸, 99%34470081132-61-23-(N-吗啡啉)丙磺酸,34583481132-61-23-(4-吗啉基)丙磺酸, 97+%30070631135-40-63-环己氨基丙烷磺酸, >98.0%(T)30352641135-40-63-(环己胺)-1-丙磺酸, 99%34467771135-40-63-(环己氨基)-1-丙磺酸,3000333115-69-52-氨基-2-甲基-1,3-丙二醇, >98.0%(GC)(T)3270840115-69-52-氨基-2-甲基-1,3-丙二醇, ≥99%3270841115-69-52-氨基-2-甲基-1,3-丙二醇, BioUltra, ≥99.5% (NT)3002661150-25-4N,N-二(2-羟乙基)甘氨酸, >99.0%(T)3029621150-25-4N,N-二(2-羟乙基)甘氨酸, 99%301524316052-06-54-(2-羟乙基)-1-哌嗪丙磺酸, >98.0%(T)304504716052-06-54-(2-羟乙基)-1-哌嗪丙磺酸, 99%344679216052-06-5N-(2-羟乙基)哌嗪-N-3-丙磺酸,300059726239-55-4N-(2-乙酰氨基)亚氨基二乙酸, >98.0%(T)302637326239-55-4N-(2-乙酰氨基)亚氨基二乙酸, 98+%333831626239-55-4乙酰氨基亚胺乙酸, ≥98% (titration)333831726239-55-4乙酰氨基亚胺乙酸, BioXtra, ≥98% (titration)333831826239-55-4乙酰氨基亚胺乙酸, VetecTM reagent grade, ≥98%333831926239-55-4乙酰氨基亚胺乙酸, BioUltra, ≥99.0% (T)344686526239-55-4N-(2-乙酰胺基)-2-亚氨基二乙酸,302375029915-38-6N-三(羟甲基)甲基-3-氨基丙烷磺酸, >98.0%(T)306011129915-38-63-[(羟甲基)甲基胺]丙烷磺酸, 99%324875029915-38-6三羟甲基甲胺基丙磺酸, ≥99.5% (titration)324875129915-38-6三羟甲基甲胺基丙磺酸, BioXtra, ≥99.5% (titration)324875229915-38-6三羟甲基甲胺基丙磺酸, VetecTM reagent grade, 99.5%324875329915-38-6三羟甲基甲胺基丙磺酸, BioPerformance Certified, cell culture tested, ≥99.5% (titration)344724929915-38-6N-三(羟甲基）甲基-3-氨基丙磺酸,30175554432-31-92-吗啉乙磺酸, >99.0%(T)31088914432-31-9MES 溶液, BioUltra, for molecular biology, 0.5 M in H2O30578145625-37-6哌嗪-1,4-双(2-乙基磺酸), 98%34468165625-37-6哌嗪-N,N-双(2-乙磺酸）,34604975625-37-61,4-哌嗪二乙磺酸, 98%30613575704-04-1N-(三羟甲基甲基)甘氨酸, 98+%32549045704-04-1三(羟甲基)甲基甘氨酸, VetecTM reagent grade, ≥99%32549055704-04-1三(羟甲基)甲基甘氨酸, BioPerformance Certified, cell culture tested, ≥99% (titration)32549065704-04-1三(羟甲基)甲基甘氨酸, ≥99% (titration)32549075704-04-1三(羟甲基)甲基甘氨酸, BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)32549085704-04-1三(羟甲基)甲基甘氨酸, anhydrous, free-flowing, Redi-DriTM, ≥99%34469135704-04-1N-三(羟甲基）甲基甘氨酸,300313964431-96-51,3-双[三(羟甲基)甲氨基]丙烷[用于缓冲材料], >99.0%(T)303034264431-96-51,3-双[三(羟甲基)甲基胺]丙烷, 98+%341533664431-96-5BIS-TRIS 丙烷, ≥99.0% (titration)341533764431-96-5BIS-TRIS 丙烷, BioXtra, ≥99.0% (titration)341533864431-96-5BIS-TRIS 丙烷, BioPerformance Certified, cell culture tested, ≥99.0%341534064431-96-5BIS-TRIS 丙烷, anhydrous, free-flowing, Redi-DriTM, ≥99.0%344677364431-96-5双[三(羟甲基）氨基丙烷],302131668189-43-5哌嗪-1,4-双(2-羟基丙烷磺酸)二水合物,344681968189-43-5哌嗪-N,N-双(2-羟基乙磺酸）,301532568399-77-92-羟基-3-吗啉基丙磺酸, >98.0%(T)305542968399-77-93-(4-吗啉基)-2-羟基丙烷磺酸, 98%344701168399-77-93-(N-吗啡啉)-2-羟基丙磺酸,301541268399-78-04-(2-羟乙基)哌嗪-1-(2-羟基丙烷-3-磺酸)水合物, >98.0%(T)344679368399-78-0N-(2-羟乙基)哌嗪-N-2-羟基丙磺酸,334187768399-79-1奎诺二甲基丙烯酯, ≥99% (titration)300353068399-80-43-[N,N-双(2-羟乙基)氨基]-2-羟基丙烷磺酸, >98.0%(HPLC)(T)302998568399-80-43-[N,N-二(羟乙基)氨基]-2-羟基丙磺酸, 99%302408668399-81-53-[N-三(羟甲基)甲氨基]-2-羟基丙磺酸, >99.0%(T)324875768399-81-53-[N-三(羟甲基)甲胺]-2-羟基丙磺酸, ≥99% (titration)324875868399-81-53-[N-三(羟甲基)甲胺]-2-羟基丙磺酸, VetecTM reagent grade, ≥99%344725168399-81-5N-三(羟甲基）甲氨酸-2-羟基丙磺酸,30035296976-37-0双(2-羟乙基)氨基三(羟甲基)甲烷, >99.0%(HPLC)(T)30299966976-37-02,2-双(羟基甲基)-2,2',2''-次氮基三乙醇, 98+%34467716976-37-0双[三(羟甲基）氨基甲烷],303526573463-39-53-(环己胺)-2-羟基-1-丙磺酸, 98%344677873463-39-53-(环己氨基)2-羟基-1-丙磺酸,30235287365-44-8N-三(羟甲基)甲基-2-氨基乙磺酸, >98.0%(T)30601767365-44-83-[(羟甲基)甲基胺]乙烷磺酸, 99%32501207365-44-8Tris 乙磺酸, ≥99% (titration)32501217365-44-8Tris 乙磺酸, BioPerformance Certified, cell culture tested, ≥99% (titration)32501227365-44-8Tris 乙磺酸, VetecTM reagent grade, 99%32501237365-44-8Tris 乙磺酸, BioXtra, ≥99.5% (titration)32501247365-44-8Tris 乙磺酸, BioUltra, ≥99.5% (calc. based on dry substance, T)32501257365-44-8Tris 乙磺酸,34470137365-44-8N-三(羟甲基）甲基-2-氨基乙磺酸,30150837365-45-92-[4-(2-羟乙基)-1-哌嗪基]乙磺酸, >99.0%(T)30494997365-45-9羟乙基哌嗪乙磺酸, 99%30637977365-45-9HEPES (晶体游离酸), 99%30958487365-45-9HEPES 缓冲液, 1 M in H2O30958737365-45-9HEPES 溶液, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture30958747365-45-9HEPES 溶液, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered34468147365-45-9N-(2-羟乙基)哌嗪-N-(2-乙磺酸),30005987365-82-4N-(2-乙酰氨基)-2-氨基乙磺酸, >99.0%(T)30259377365-82-4N-(2-乙酰氨基)-2氨基乙磺酸, 99%33371907365-82-4N-氨基甲酰甲基乙磺酸, ≥99.0% (titration)33371917365-82-4N-氨基甲酰甲基乙磺酸, BioPerformance Certified, ≥99.0%33371927365-82-4N-氨基甲酰甲基乙磺酸, VetecTM reagent grade, 98%33371937365-82-4N-氨基甲酰甲基乙磺酸, BioXtra, ≥99.0% (titration)33371947365-82-4N-氨基甲酰甲基乙磺酸, BioUltra, ≥99.5% (T)33371957365-82-4N-氨基甲酰甲基乙磺酸, anhydrous, free-flowing, Redi-DriTM, ≥99.0%34468207365-82-4N-(2-乙酰氨基）-2-氨基乙烷磺酸,300032477-86-1三（羟甲基）氨基甲烷, >99.0%(T)302493977-86-1三(羟甲基)氨基甲烷[用于电泳], >99.0%(T)306248577-86-1三(羟甲基)氨基甲烷, ACS,99.8-100.1% (Assay,dried basis)306248677-86-1三(羟甲基)氨基甲烷, 99%325604477-86-1三(羟基甲基)氨基甲烷, ACS reagent, ≥99.8%325621377-86-1Trizma 碱, anhydrous, free-flowing, Redi-DriTM, ≥99.9%325621477-86-1Trizma 碱, Primary Standard and Buffer, ≥99.9% (titration), crystalline325621577-86-1Trizma 碱, VetecTM reagent grade, ≥99%325621777-86-1Trizma 碱, BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)325621877-86-1Trizma 碱, ≥99.9% (titration), crystalline325621977-86-1Trizma 碱, BioUltra, for molecular biology, ≥99.8% (T)325622077-86-1Trizma 碱, ≥99.0% (T)325622177-86-1Trizma 碱, puriss. p.a., ≥99.7% (T)325622277-86-1Trizma 碱, puriss. p.a., buffer substance, ≥99.5%325622377-86-1Trizma 碱, tested according to Ph.Eur.325622577-86-1Trizma 碱, cell culture tested, ≥99.9% (titration), crystalline325632477-86-1氨基丁三醇, European Pharmacopoeia (EP) Reference Standard325632577-86-1缓血酸胺, meets USP testing specifications325632677-86-1缓血酸胺, pharmaceutical secondary standard; traceable to USP and PhEur344672177-86-1三羟甲基氨基甲烷,